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Your Position: Home > Protein > CD229 > CD9-H52H6

Human CD229 / SLAMF3 Protein, His Tag

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  • Synonym
    SLAMF3,LY9,Ly-9,hly9,Ly9
  • Source
    Human CD229, His Tag(CD9-H52H6) is expressed from human 293 cells (HEK293). It contains AA Lys 48 - Lys 454 (Accession # Q9HBG7-1).
    Predicted N-terminus: Lys 48
  • Molecular Characterization
    CD229 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 46.7 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD229 SDS-PAGE

Human CD229, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD229 ELISA

Immobilized Human CD229, His Tag (Cat. No. CD9-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD229, His,Avitag, premium grade (Cat. No. CD9-H82E6) with a linear range of 0.039-2.5 μg/mL (QC tested).

  • Background
    CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Signaling lymphocyte activation molecule (SLAM) family receptors are critically involved in modulating innate and adaptive immune responses. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig-like domain.
  • Clinical and Translational Updates

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