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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIB / CD32b > Mouse Fc gamma RIIB / CD32b Protein (SPR verified)

Mouse Fc gamma RIIB / CD32b Protein (SPR verified)

  • Synonym
    FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32
  • Source
    Mouse CD32b (SPR verified) (CDB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Thr 40 - Arg 217 (Accession # NP_001070657).
    Predicted N-terminus: Thr 40
  • Molecular Characterization
    Online(Thr 40 - Arg 217) NP_001070657

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD32b (SPR verified) (Cat. No. CDB-M52H7) SDS-PAGE gel

Mouse CD32b (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Mouse CD32b (Cat. No. CDB-M52H7) on NTA-Series S sensor chip , can bind OKT3 with an affinity constant of 0.86 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b (Cat. No. CDB-M52H7) with an affinity constant of 0.55 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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