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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-H52Hc

Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (MALS verified)

  • Synonym
    CD28,Tp44
  • Source
    Human / Cynomolgus / Rhesus macaque CD28, His Tag (CD8-H52Hc) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) P10747-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 17.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. ) MALS images

The purity of Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) was more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human / Cynomolgus / Rhesus macaque CD28, His TagHuman / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) ELISA bioactivity

Immobilized Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with a linear range of 0.1-1 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 5.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human B7-2, Fc Tag (Cat. No. CD6-H5257) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28, His Tag (Cat. No. CD8-H52Hc) with an affinity constant of 0.169 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $350.00

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DMF (Drug Master File)

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