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Your Position: Home > All Other Proteins > APRIL > APL-H5244

Human APRIL / TNFSF13 Protein, His Tag

  • Synonym
    APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2
  • Source
    Human APRIL, His Tag (APL-H5244) is expressed from human 293 cells (HEK293). It contains AA Ala 105 - Leu 250 (Accession # O75888-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Ala 105 - Leu 250) O75888-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 22.8 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human APRIL, His Tag (Cat. No. APL-H5244) SDS-PAGE gel

Human APRIL, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human APRIL, His TagHuman APRIL, His Tag (Cat. No. APL-H5244) ELISA bioactivity

Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 5 μg/mL (100 μL/well) can bind Human APRIL, His Tag (Cat. No. APL-H5244) with a linear range of 8-125 ng/mL (QC tested).

Human APRIL, His TagHuman APRIL, His Tag (Cat. No. APL-H5244) ELISA bioactivity

Immobilized Human APRIL, His Tag (Cat. No. APL-H5244) at 1 μg/mL (100 μL/well) can bind Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) with a linear range of 3-25 ng/mL (Routinely tested).

  • Background
    APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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