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Your Position: Home > Immune Checkpoint Proteins > B7-H3 (4Ig) > B7B-H82F5

Biotinylated Human B7-H3 (4Ig) / B7-H3b Protein, Fc,Avitag™

  • Synonym
    4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309/PRO352,B7 homolog 3
  • Source
    Biotinylated Human B7-H3 (4Ig), Fc,Avitag (B7B-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).
    Predicted N-terminus: Gly 27
  • Molecular Characterization
    Online(Gly 27 - Thr 461) Q5ZPR3-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) SDS-PAGE gel

Biotinylated Human B7-H3 (4Ig), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human B7-H3 (4Ig), Fc,AvitagBiotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) ELISA bioactivity

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) with a linear range of 0.3-2 ng/mL (QC tested).

Biotinylated Human B7-H3 (4Ig), Fc,AvitagBiotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) ELISA bioactivity

Immobilized Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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