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Your Position: Home > All Other Proteins > DPPIV > DP4-H5211

Human DPPIV / CD26 Protein, Tag Free (Active dimer) (active enzyme)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    DPP4,ADABP,ADCP2,CD26,DPPIV,TP103
  • Source
    Human DPPIV, Tag Free (DP4-H5211) is expressed from human 293 cells (HEK293). It contains AA Asn 29 - Pro 766 (Accession # NP_001926.2).
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  • Molecular Characterization
    Online(Asn 29 - Pro 766) NP_001926.2

    This protein carries no "tag".

    The protein has a calculated MW of 85.5 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human DPPIV, Tag Free (Cat. No. DP4-H5211) SDS-PAGE gel

Human DPPIV, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human DPPIV, Tag Free (Cat. No. ) MALS images

The purity of Human DPPIV, Tag Free (Cat. No. DP4-H5211) was more than 95% and the molecular weight of this protein is around 190-220 kDa verified by SEC-MALS.

Report
Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >11000 pmol/min/µg (QC tested).

Protocol
Please do not use together with anti human IgG Fc secondary antibody in order to avoid high background.
  • Background
    Dipeptidyl peptidase-IV (DPPIV) is also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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