This protein carries no "tag"
The protein has a calculated MW of 12.9 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 25 mM Histidine, pH6.2 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-15, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-15, premium grade (Cat. No. IL5-H4117) at 2 μg/mL (100 μL/well) can bind Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253) with a linear range of 0.6-39 ng/mL (Routinely tested).
Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 9.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) on Protein A Biosensor, can bind Human IL-15, premium grade (Cat. No. IL5-H4117) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human IL-15, premium grade (Cat. No. IL5-H4117) stimulates proliferation of CTLL-2 cells. The EC50 for this effect is 0.9946 ng/mL, corresponding to a specific activity of > 0.8 ⅹ10^7 IU/mg, which is calibrated against human IL-15 WHO International Standard (NIBSC code: 95/554) (QC tested).
Human IL-15, premium grade (Cat. No. IL5-H4117), designed for preclinical stage, has the same activity and performance with GMP Human IL-15 (Cat. No. GMP-L15H13), which enables a seamless transition from preclinical development to clinical phases.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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