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Your Position: Home > ActiveMax® Cytokines > Human EPO /Erythropoietin Protein,HEK293 expressed,ActiveMax

ActiveMax® Recombinant Human Erythropoietin / EPO

  • Synonym
    EPO,EP,MVCD2,Erythropoietin,Erythropoetin,Erthropoyetin,Hematopoietin,Hemopoietin
  • Source
    ActiveMax® Human EPO (EPO-H4214) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Arg 193 (Accession # AAH93628.1).
    Predicted N-terminus: Ala 28
  • Molecular Characterization
    EPO(Ala 28 - Arg 193) AAH93628.1

    This protein carries no "tag".

    The protein has a calculated MW of 18.4 kDa. The protein migrates as 30-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >97% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human EPO (Cat. No. EPO-H4214) SDS-PAGE gel

ActiveMax® Human EPO on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity
ActiveMax® Human EPOActiveMax® Human EPO (Cat. No. EPO-H4214) ELISA bioactivity

Immobilized Human EPO, Tag Free (Cat. No. EPO-H4214) at 10μg/mL (100 μL/well) can bind Human EPO R, Fc Tag (Cat. No. EPR-H5253) with a linear range of 0.013-0.12 μg/mL (Routinely tested) .

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