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Your Position: Home > All Other Proteins > Mesothelin > GFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

GFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    GFP Fusion Human Mesothelin (296-580), His Tag (MSN-HG2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
    Predicted N-terminus: Ala 2
  • Molecular Characterization
    Online(Glu 296 - Gly 580) AAH09272.1

    This protein carries a GFP tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.4 kDa. The protein migrates as 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Application
    Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc.
  • Conjugate

    GFP

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 530 nm

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) SDS-PAGE gel

GFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
GFP Fusion Human Mesothelin (296-580), His TagGFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) ELISA bioactivity

Immobilized Anti-Human MSLN MAb at 10 μg/mL (100 μL/well) can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with a linear range of 0.039-0.625 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4) with an affinity constant of 0.542 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of the MSLN-CAR-293 cells were stained with 100ul of 3 μg/mL of GFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HG2P4). GFP was used as negative control (Routinely tested).

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