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Your Position: Home > All Other Proteins > Siglec-15 > Human Siglec-15 / CD33L3 Protein, His Tag

Human Siglec-15 / CD33L3 Protein, His Tag

  • Synonym
    CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15
  • Source
    Human Siglec-15, His Tag (SG5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
    Predicted N-terminus: Phe 20
  • Molecular Characterization
    Online(Phe 20 - Thr 263) Q6ZMC9-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.4 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-15, His Tag (Cat. No. SG5-H52H3) SDS-PAGE gel

Human Siglec-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Siglec-15, His TagHuman Siglec-15, His Tag (Cat. No. SG5-H52H3) ELISA bioactivity

Immobilized Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.3-2 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Mouse Human chimeric MAb (5G12, Human IgG1) on AHC Biosensor, can bind Human Siglec-15, His Tag (Cat. No. SG5-H52H3) with an affinity constant of 0.52 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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