A-Z> All Other Proteins > Mesothelin > RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag 
This protein carries a RFP tag at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
RFP
Excitation source: 561 nm spectral line, Yellow Green Laser
Excitation Wavelength: 561 nm
Emission Wavelength: 586/15 nm
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
RFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Human MSLN Mab, Human IgG1 at 10 μg/mL (100 μL/well) can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with a linear range of 0.078-0.625 μg/mL (QC tested).
Anti-human MSLN MAb (chimeric mouse-human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface, can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with an affinity constant of 0.304 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4). Non-transfected 293 cells were used as negative control (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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