This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
RFP
Excitation source: 561 nm spectral line, Yellow Green Laser
Excitation Wavelength: 561 nm
Emission Wavelength: 586/15 nm
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
RFP Fusion Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
can bind Human MS4A1 / CD20 Full Length Protein, His Tag (Cat. No. CD0-H55Ha)with an affinity constant of 1.21 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4). Non-transfected 293 cells were used as negative control (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
ACROBiosystems has developed an exclusive immune checkpoint protein collection， The consistency and excellent performance of our proteins are confirmed by a variety of assays.
ACROBiosystems has developed a series of homogeneous CD3 proteins with good bioactivity. They are verified as 1:1 heterodimers by nonreductive electrophoresis and MALS as well.
ACROBiosystems has developed a series of products with different species and tags for a variety of hot targets. It is suitable for immune, antibody screening, SPR, cell activity detection and other experiments, and all SPR&BLI protocols are available for free.
We also supply difficult-to-express proteins such as BCMA, CD19, ROR1, and EGFRVIII.
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