This protein carries no "tag".
The protein has a calculated MW of 14.5 kDa. The protein migrates as 18-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
ActiveMax® Human GM-CSF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.1 ng/mL, corresponding to a specific activity of > 1x107 Unit/mg.
Authors: Yi, S., et al.
Journal: Immunology and Microbiology 43325
Application: Cell Culture
Authors: He Y, et al.
Journal: Br J Ophthalmol 2018
Application: Cell Culture
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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