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Your Position: Home > Cytokines > GM-CSF > ActiveMax® Human GM-CSF Protein, Tag Free

ActiveMax® Human GM-CSF Protein, Tag Free

  • Synonym
    GM-CSF,CSF2,MGC131935
  • Source
    ActiveMax® Human GM-CSF, Tag Free (GMF-H4214) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2).
    Predicted N-terminus: Ala 18
  • Molecular Characterization
    Online(Ala 18 - Glu 144) NP_000749.2

    This protein carries no "tag".

    The protein has a calculated MW of 14.5 kDa. The protein migrates as 18-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ActiveMax® Human GM-CSF, Tag Free (Cat. No. GMF-H4214) SDS-PAGE gel

ActiveMax® Human GM-CSF, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.1 ng/mL, corresponding to a specific activity of > 1x107 Unit/mg.

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