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Your Position: Home > Receptor Tyrosine Kinases > Insulin R > INR-H52Ha

Human Insulin R / CD220 (28-944) Protein, His Tag (MALS & SPR verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    INSR,Insulin receptor,IR,CD220
  • Source
    Human Insulin R (28-944), His Tag (INR-H52Ha) is expressed from human 293 cells (HEK293). It contains AA His 28 - Lys 944 (Accession # P06213-2).
    Predicted N-terminus: His 28
    Request for sequence
  • Molecular Characterization
    Online(His 28 - Lys 944) P06213-2

    This protein carries a polyhistidine tag at the C-terminus. The mature form of Insulin R a disulfide-linked heterotetramer composed of 2 alpha and 2 beta chains. Each α and β chain (partial fragment) has a calculated MW of 83.6 kDa and 22.8 kDa. The protein migrates as 120-130 kDa and 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) SDS-PAGE gel

Human Insulin R (28-944), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human Insulin R (28-944), His Tag (Cat. No. ) MALS images

The purity of Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) is more than 90% and the molecular weight of this protein is around 256-313 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
Human Insulin R (28-944), His TagHuman Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) ELISA bioactivity

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Human IGF-II, Fc Tag (Cat. No. IG2-H4260) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol
Human Insulin R (28-944), His TagHuman Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) ELISA bioactivity

Immobilized Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) at 5 μg/mL (100 μL/well)can bind Insulin Biotin Conjugate with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Protocol
Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Insulin R (28-944), His Tag (Cat. No. INR-H52Ha) captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nM as determined in a SPR assay (Biacore T200) (QC tested).

Protocol
  • Background
    Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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