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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > Biotinylated Mouse CD27 Ligand / CD70 Protein, His Tag, Avi Tag

Biotinylated Mouse CD27 Ligand / CD70 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Biotinylated Mouse CD27 Ligand, His Tag, Avi Tag (CDL-M82Qb) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Gln 47 - Pro 195) O55237-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 20.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Mouse CD27 Ligand, His Tag, Avi Tag (Cat. No. CDL-M82Qb) SDS-PAGE gel

Biotinylated Mouse CD27 Ligand, His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Mouse CD27 Ligand, His Tag, Avi TagBiotinylated Mouse CD27 Ligand, His Tag, Avi Tag (Cat. No. CDL-M82Qb) ELISA bioactivity

Immobilized Mouse CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well)can bind Biotinylated Mouse CD27 Ligand, His Tag, Avi Tag (Cat. No. CDL-M82Qb) with a linear range of 0.1-3 ng/mL (QC tested).

  • Background
    Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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  • PEGS Boston 2018
  • WPC Boston 2018

CD27 Ligand Products

  • Name:CD70 antigen
  • Classification:Homo sapiens
  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:7 Details
  • Lastest Research Phase:Phase ?
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