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Your Position: Home > All Other Proteins > IL-2 R alpha > ILA-H82E6

Biotinylated Human IL-2 R alpha / CD25 Protein, His,Avitag™

  • Synonym
    IL2RA,CD25,p55,IL2-RA,IL-2-RA
  • Source
    Biotinylated Human IL-2 R alpha, His,Avitag (ILA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # P01589-1).
    Predicted N-terminus: Glu 22
  • Molecular Characterization
    Online(Glu 22 - Cys 213) P01589-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) SDS-PAGE gel

Biotinylated Human IL-2 R alpha, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human IL-2 R alpha, His,AvitagBiotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) ELISA bioactivity

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4113) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) with a linear range of 10-156 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) on SA Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 70.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Biotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.57-0.81 µg/mL (Routinely tested).

  • Background
    Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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