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Your Position: Home > Protein > CD3E & CD3D > CDD-C5259

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

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  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon
  • Source
    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin(CDD-C5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)).
    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D Structure

    Cynomolgus CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50 kDa and 55-65 kDa due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD3E & CD3D SDS-PAGE

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD3E & CD3D ELISA

Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-C5259) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 2-63 ng/mL (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $368.00

Price(USD) : $2667.00

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