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Your Position: Home > Protein > CD3E & CD3D > CDD-H5258

Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

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  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon
  • Source
    Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin(CDD-H5258) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D Structure

    Human CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50-60 kDa due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD3E & CD3D SDS-PAGE

Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 CD3E & CD3D ELISA

Immobilized Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-H5258) at 2 μg/mL (100 μL/well) can bind SP34-2 (mouse IgG1) with a linear range of 1-20 ng/mL (Routinely tested).

  • Clinical and Translational Updates

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Price(USD) : $350.00

Price(USD) : $2540.00

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