>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 years under sterile conditions after reconstitution.
Anti-Trastuzumab Antibodies on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Anti-Trastuzumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized trastuzumab at 1 µg/ml, added increasing concentrations of Anti-Trastuzumab Antibodies (Cat. No. TRB-Y7, 10% human serum) and then added biotinylated trastuzumab at 2 µg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 19 ng/mL.
Demonstration of the specificity of Anti-Trastuzumab Antibodies (Cat. No. TRB-Y7) to the trastuzumab.
Measured by its neutralizing ability in a functional ELISA. Immobilized trastuzumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Trastuzumab Antibodies (Cat. No. TRB-Y7) and Biotinylated Human HER2 (Cat. No. HE2-H82E2) with a inhibition rate of 89%.
Anti-Trastuzumab Antibodies (mouse IgG1, Cat. No. TRB-Y7) captured on CM5 chip via anti-mouse antibodies surface, can bind human trastuzumab with an affinity constant of 0.452 nM.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $300.00
Price(USD) : $1980.00
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