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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-C5255

Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-C5255) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)).
    Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)) Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

    Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 37.3 kDa (CD3E) and 37.0 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-45 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5255) SDS-PAGE gel

Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc TagCynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5255) ELISA bioactivity

Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5255) with a linear range of 0.3-2 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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