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Your Position: Home > Fc Receptor Proteins > Fc gamma RI / CD64 > FCA-C82E8

Biotinylated Cynomolgus Fc gamma RI / CD64 Protein, His,Avitag™ (BLI verified)

  • Synonym
    FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI
  • Source
    Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (FCA-C82E8) is expressed from human 293 cells (HEK293). It contains AA Val 11 - Pro 288 (Accession # NP_001270969.1).
    Predicted N-terminus: Val 11
  • Molecular Characterization
    Online(Val 11 - Pro 288) NP_001270969.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 34.8 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (Cat. No. FCA-C82E8) SDS-PAGE gel

Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Cynomolgus CD64, His,Avitag (BLI verified)Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (Cat. No. FCA-C82E8) ELISA bioactivity

Immobilized FMC63 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (Cat. No. FCA-C82E8) with a linear range of 0.2-10 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus CD64, His,Avitag (BLI verified) (Cat. No.FCA-C82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.326 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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