This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.2 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>85% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD20 Full Length, His Tag (HEK293) (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with a linear range of 0.4-3 ng/mL (in presence of DDM and CHS) (QC tested).
MabThera® (Rituximab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with an affinity constant of 6.21 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).
Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) captured on CM5 chip via Anti-His antibody can bind MabThera® (Rituximab) with an affinity constant of 0.921 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biosimilar of Obinutuzumab captured on CM5 chip via anti-human IgG Fc antibody can bind Human CD20 Full Length, His Tag (HEK293) (SPR verified) (Cat. No. CD0-H52H3) with an affinity constant of 21.5 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Ofatumumab captured on CM5 chip via anti-human IgG Fc antibody can bind Human CD20 Full Length, His Tag (HEK293) (SPR verified) (Cat. No. CD0-H52H3) with an affinity constant of 4.47 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Loaded Rituximab on AHC Biosensor, can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Ofatumumab (Human IgG1) on AHC Biosensor, can bind Human CD20 Full Length, His Tag, HEK293 (SPR verified) (Cat. No. CD0-H52H3) with an affinity constant of 0.968 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $850.00
Price(USD) : $2990.00
Price(USD) : $7480.00
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