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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-C82W6

Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (CDD-C82W6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1(CD3E) & Q95LI8-1(CD3D)).
    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)) Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

    Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 17.8 kDa (CD3E) and 13.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and followed by an Avi tag (Avitag™) and subunit CD3D contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 21-23 kDa (CD3E) and 19-21 kDa (CD3D) due to glyosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (Cat. No. CDD-C82W6) SDS-PAGE gel

Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (Cat. No. ) MALS images

The purity of Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (MALS verified) (Cat. No.CDD-C82W6) was more than 90% and the molecular weight of this protein is around 38-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag FreeBiotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (Cat. No. CDD-C82W6) ELISA bioactivity

Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (Cat. No. CDD-C82W6) with a linear range of 0.5-3.9 ng/mL (Intact assay, QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (Cat. No. CDD-C82W6) with an affinity constant of 53.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) on SA Biosensor, can bind Human CD3×BCMA scFV with an affinity constant of 1.84 nM as determined in BLI assay (ForteBio Octet Red9e) (Routinely tested).

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