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Your Position: Home > Cytokines > TRAIL > TRL-H5259

Human TRAIL / TNFRSF10 Protein, Mouse IgG2a Fc Tag

  • Synonym
    Apo-2L,TRAIL,CD253,TNFSF10,Apo-2 ligand
  • Source
    Human TRAIL, Mouse IgG2a Fc Tag (TRL-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 114 - Gly 281 (Accession # P50591-1).
    Predicted N-terminus: Glu
  • Molecular Characterization
    Online(Val 114 - Gly 281) P50591-1

    This protein carries a mouse IgG2a Fc tag at the N-terminus.

    The protein has a calculated MW of 46.4 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) SDS-PAGE gel

Human TRAIL, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human TRAIL, Mouse IgG2a Fc TagHuman TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) ELISA bioactivity

Immobilized Human Osteoprotegerin, His Tag (Cat. No. TNB-H5220) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 0.8-13 ng/mL (QC tested).

Human TRAIL, Mouse IgG2a Fc TagHuman TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) ELISA bioactivity

Immobilized Human TRAIL R1, His Tag (Cat. No. TR1-H5228) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 2-20 ng/mL (Routinely tested).

Human TRAIL, Mouse IgG2a Fc TagHuman TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) ELISA bioactivity

Immobilized Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL R2, Fc Tag (Cat. No. TR2-H5255) with a linear range of 0.6-20 ng/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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