0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antibodies > Adalimumab > Anti-Adalimumab Antibodies (recommended for PK/PD)

Anti-Adalimumab Antibodies (recommended for PK/PD)

  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab F(ab')2.
  • Isotype
    IgG1/kappa
  • Specificity
    Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 years under sterile conditions after reconstitution.
SDS-PAGE
Anti-Adalimumab Antibodies (recommended for PK/PD) (Cat. No. ADB-Y23) SDS-PAGE gel

Anti-Adalimumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Anti-Adalimumab Antibodies (recommended for PK/PD)Anti-Adalimumab Antibodies (recommended for PK/PD) (Cat. No. ADB-Y23) ELISA bioactivity

Detection of adalimumab by bridging ELISA in serum. Immobilized Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) at 2 μg/mL, add increasing concentrations of adalimumab (10% human serum) and then add Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 2 ng/mL.

Anti-Adalimumab Antibodies (recommended for PK/PD)Anti-Adalimumab Antibodies (recommended for PK/PD) (Cat. No. ADB-Y23) ELISA bioactivity

Demonstration of the specificity of Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) to the adalimumab.

Anti-Adalimumab Antibodies (recommended for PK/PD)Anti-Adalimumab Antibodies (recommended for PK/PD) (Cat. No. ADB-Y23) ELISA bioactivity

Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) and Biotinylated Human TNF-alpha, His Tag (Cat. No. TNA-H82E3) with a inhibition rate of 8%.

  • Background
    Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

Promotion & Exhibitions

  • PEGS Boston 2019
  • PepTalk 2019

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop