>Biotinylated Anti-Trastuzumab Antibodies (recommended for PK/PD)
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Anti-Trastuzumab Antibodies (recommended for PK/PD) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Detection of trastuzumab by bridging ELISA in serum. Immobilized Anti-Trastuzumab Antibodies (Cat. No. TRB-Y1) at 2 μg/ml, added increasing concentrations of trastuzumab (10% human serum) and then added Biotinylated Anti-Trastuzumab Antibodies (Cat. No. TRB-BY5) at 2 μg/ml. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/ml.
Immobilized trastuzumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Trastuzumab Antibodies (Cat. No. TRB-BY5) with a linear range of 0.78-25 ng/mL.
ELISA analysis shows that the binding of trastuzumab to Human Her2, His Tag (Cat. No. HE2-H5225) was inhibited by increasing concentration of Biotinylated Anti-Trastuzumab Antibodies (Cat. No. TRB-BY5). The concentration of trastuzumab used is 12.5 ng/ml. The IC50 is 13.67 μg/ml.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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