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Your Position: Home > Cytokines > HGF > HGF-H52H3

Human HGF Protein, His Tag

  • Synonym
    HGF,HPTA,SF
  • Source
    Human HGF, His Tag (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Online(Gln 32 - Ser 728) P14210-1

    This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90kDa (alpha & Beta chain), 58-63 (alpha chain), 33 kDa (Beta chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human HGF, His Tag (Cat. No. HGF-H52H3) SDS-PAGE gel

Human HGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human HGF, His TagHuman HGF, His Tag (Cat. No. HGF-H52H3) ELISA bioactivity

Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 1-39 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human HGF R Protein, Fc Tag (Cat. No. MET-H5256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF Protein, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 75.2 pM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human HGF R, Fc Tag (Cat. No. MET-H5256) on Protein A Biosensor, can bind Human HGF, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $400.00

Price(USD) : $2600.00

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DMF (Drug Master File)

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