Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-55 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (Cat. No. FCM-M82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 2.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified)(Cat. No. FCM-M82W5) on SA Biosensor, can bind Mouse Serum Albumin, His Tag(Cat. No. MSA-M52H8) with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
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