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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCM-M82W5

Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (BLI verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (FCM-M82W5) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1(FCGRT) & P01887-1(B2M)).
    Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)) Q61559-1 (FCGRT) & P01887-1 (B2M)

    Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-55 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (Cat. No. FCM-M82W5) SDS-PAGE gel

Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified)Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (Cat. No. FCM-M82W5) ELISA bioactivity

Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (Cat. No. FCM-M82W5) with a linear range of 5-78 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified) (Cat. No. FCM-M82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 2.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (BLI verified)(Cat. No. FCM-M82W5) on SA Biosensor, can bind  Mouse Serum Albumin, His Tag(Cat. No. MSA-M52H8)  with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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