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Your Position: Home > Cytokines > IL-4 > Human IL-4 /Interleukin-4 Protein,HEK293 expressed,ActiveMax

ActiveMax® Recombinant Human IL-4

  • Synonym
    IL4,BCGF1,BSF1
  • Source
    Human IL-4, Tag Free (IL4-H4218) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # AAH67514).
    Predicted N-terminus: His 25
  • Molecular Characterization
    Online(His 25 - Ser 153) AAH67514

    This protein carries no "tag".

    The protein has a calculated MW of 14.9 kDa. The protein migrates as 15 and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS and NaCl. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-4, Tag Free (Cat. No. IL4-H4218) SDS-PAGE gel

Human IL-4, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human IL-4, Tag FreeHuman IL-4, Tag Free (Cat. No. IL4-H4218) ELISA bioactivity

Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, His Tag (Cat. No. ILR-H82E9) with a linear range of 2-78 ng/mL (QC tested).

Human IL-4, Tag FreeHuman IL-4, Tag Free (Cat. No. IL4-H4218) ELISA bioactivity

Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) with a linear range of 1-20 ng/mL (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL-4, Tag Free (Cat. No. IL4-H4218) stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.27-0.49 ng/ml (Routinely tested).

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Order Details

Price(USD) : $180.00

Price(USD) : $400.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Drug Development Status

  • Number of Approved Drugs:0 Details
  • Number of Drugs in Clinical Trials:6 Details
  • Latest Clinical Research Stage:Phase ?

Datasheet & Documentation

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