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Your Position: Home > All Other Proteins > CD133 > CD3-H55H7

Human CD133 Protein, His Tag (InsectCells)

  • Synonym
    CD133,PROM1,PROML1,Prominin-1,AC133
  • Source
    Human CD133, His Tag (CD3-H55H7) is expressed from Baculovirus-Insect cells. It contains AA Gly 20 - His 865 (Accession # O43490-1).
    Predicted N-terminus: Gly 20
  • Molecular Characterization
    Online(Gly 20 - His 865) O43490-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 97.2 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >80% as determined by SDS-PAGE.

  • Formulation

    Delivered as bulk protein in a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep CD133 soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;

    2. -70°C for 3 months under sterile conditions.

  • Shipping
    This product is supplied as sterile liquid solution and shipped frozen with dry ice, please inquire the shipping cost.
SDS-PAGE
Human CD133, His Tag (Cat. No. CD3-H55H7) SDS-PAGE gel

Human CD133, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.

Bioactivity-ELISA
Human CD133, His TagHuman CD133, His Tag (Cat. No. CD3-H55H7) ELISA bioactivity

Immobilized Human CD133, His Tag (Cat. No. CD3-H55H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD133 (133-3) Antibody, Human IgG1 with a linear range of 0.4-16 ng/mL (QC tested).

  • Background
    Prominin-1 is also known as CD133, Antigen AC133, PROM1, PROML1 and MSTP061. Is used as marker for hematopoietic stem and progenitor cells (HSPC) for somatic stem cell isolation. May play a role in cell differentiation, proliferation and apoptosis . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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