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Your Position: Home > All Other Proteins > CD4 > CD4-HF255

FITC-Labeled Human CD4 Protein, Fc Tag

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    FITC-Labeled Human CD4, Fc Tag (CD4-HF255) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782). It is the FITC labeled form of Human CD4, Fc Tag (CD4-H5259).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    Online(Lys 26 - Pro 396) AAH25782

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 67.7 kDa. The protein migrates as 67-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 3-5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) SDS-PAGE gel

FITC-Labeled Human CD4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
FITC-Labeled Human CD4, Fc TagFITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) ELISA bioactivity

Immobilized Ibalizumab (Human IgG4) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) with a linear range of 20-313 ng/mL (QC tested).

FITC-Labeled Human CD4, Fc TagFITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255) ELISA bioactivity

Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD4, Fc Tag (Cat. No. CD4-HF255)) with a linear range of 0.04-0.313 μg/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of CD4-CAR-293 cells transfected with anti-CD4-scFv were stained with 100 μL of 1 μg/mL of FITC-Labeled Human CD4 Protein, Fc Tag (Cat. No. CD4-HF255) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).

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Price(USD) : $319.00

Price(USD) : $1980.00

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