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Your Position: Home > All Other Proteins > FGL1 > FG1-H82Ey

Biotinylated Human FGL1 Protein, His,Avitag™ (recommended for biopanning)

  • Synonym
    FGL1,Hepassocin,HP-041,HFREP-1,LFIRE-1,HFREP1
  • Source
    Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (FG1-H82Ey) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Ile 312 (Accession # Q08830-1).
  • Molecular Characterization
    Online(Leu 23 - Ile 312) Q08830-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 37.9 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) SDS-PAGE gel

Biotinylated Human FGL1, His,Avitag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human FGL1, His,Avitag (recommended for biopanning)Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) ELISA bioactivity

Immobilized Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Anti-FGL1 antibody with a linear range of 0.01-0.625 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) on SA Biosensor, can bind Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) with an affinity constant of 8.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Biotinylated Human FGL1, His,Avitag (Cat. No. FG1-H82Ey) can bind to 293T cells overexpressing Human LAG3. The concentration of Human FGL1 is 10 μg/mL (Routinely tested).

  • Background
    Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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