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Your Position: Home > Immune Checkpoint Proteins > PD-1 > PD1-C82E6

Biotinylated Cynomolgus PD-1 / PDCD1 Protein, His,Avitag™

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Biotinylated Cynomolgus PD-1, His,Avitag (PD1-C82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # B0LAJ3).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) B0LAJ3

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 19.5 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) SDS-PAGE gel

Biotinylated Cynomolgus PD-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Cynomolgus PD-1, His,AvitagBiotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) ELISA bioactivity

Immobilized Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Cynomolgus / Rhesus macaque PD-L1, Fc Tag (Cat. No. PD1-C5253) with a linear range of 0.2-5 ng/mL (QC tested).

Biotinylated Cynomolgus PD-1, His,AvitagBiotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) ELISA bioactivity

Immobilized Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Nivolumab with a linear range of 1-20 ng/mL (Routinely tested).

Biotinylated Cynomolgus PD-1, His,AvitagBiotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) ELISA bioactivity

Immobilized Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) with a linear range of 0.039-1.25 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) on SA Biosensor, can bind Cynomolgus / Rhesus macaque PD-L1, His Tag (Cat. No. PD1-C52H4) with an affinity constant of 2.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus PD-1, His,Avitag (Cat. No. PD1-C82E6) on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.49 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:6 Details
  • Number of Drugs in Clinical Trials:75 Details
  • Latest Research Phase:Approved

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