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Your Position: Home > MabAffinity Chromatography Media > MabAffinity Protein A Agarose High Flow, 25ml

MabAffinity rProtein A High Flow Beads,25ml

MabAffinity rProtein A High Flow Beads is a classic protein A affinity medium for fast and efficient purification of monoclonal and polyclonal antibodies at any scale. The high specificity of rProtein A ligand to Fc domain of IgGs gives a high purification factor in a single chromatography step. Superior coupling chemistry ensures both high capacity and low leakage.
Features
  1. Classic rProtein A resin suitable for different applications, including purification of poly or monoclonal antibodies of different species and subclasses and isolation of immune complex

  2. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification
CompositionHighly cross-linked Agarose
Average particle size90 micron
FormSlurry in 20% Ethanol
Pack size25ml settled agarose media
(50ml 50% slurry)
LigandrProtein A
Coupling chemistryEpoxy
Dynamic binding capacity1> 30mg IgG /ml media
Recommended flow rate100-300cm/hr
Chemical stabilityStable in all aqueous buffers commonly used in protein A chromatography
pH working range3-10
pH CIP range (short term)2-11
CIP stability8M Urea, 6M Gua-HCl or 10mM NaOH+1M NaCl, 0.1M acetic acid
Temperature stability22-40oC
Storage20% Ethanol
Shelf life5 years
  1. Determined at 10% breakthrough at 4min residence time

  2. Delivered at room temperature, and recommended long-term storage at 2-8 oC

MabAffinity rProtein A High Flow Beads belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity rProtein A High Flow Beads are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

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Order Details

Price(USD) : $635.00

Inventory : In stock

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