This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 87.5 kDa. The protein migrates as 150-200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human 4-1BB Ligand (71-254), Fc Tag, active trimer on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) is more than 90% in HP-SEC, and the molecular weight of this protein is around 185-225 kDa verified by SEC-MALS.
Loaded Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) with an affinity constant of 0.103 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $2310.00
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