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Your Position: Home > Protein > CD52 > CD2-H82E8

Biotinylated Human CD52 Protein, His,Avitag™

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  • Synonym
    CDW52,HE5,CD52,CAMPATH-1 antigen
  • Source
    Biotinylated Human CD52, His,Avitag(CD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ser 36 (Accession # P31358-1).
  • Molecular Characterization
    CD52 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 13.5 kDa. The protein migrates as 16 kDa and 17-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD52 SDS-PAGE

Biotinylated Human CD52, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CD52 ELISA

Immobilized Monoclonal Anti-Human CD52 Antibody, Human IgG1 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) with a linear range of 0.005-0.625 μg/mL (QC tested).

Bioactivity-SPR
 CD52 SPR

Anti-Human CD52 MAb (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) with an affinity constant of 71.8 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 CD52 BLI

Loaded Biotinylated Human CD52, His,Avitag (Cat. No. CD2-H82E8) on SA Biosensor, can bind Anti-Human CD52 MAb (Human IgG1) with an affinity constant of 137 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene.It is widely expressed on the cell surface of immune cells, such as mature lymphocytes, natural killer cells (NK), eosinophils, neutrophils, monocytes/macrophages, and dendritic cells (DCs).ligation of cell surface CD52 molecules may offer costimulatory signals for T-cell activation and proliferation.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Approved

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