Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified) is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.2 kDa (CD3E) and 37.3 kDa (CD3D). Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with Flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) protein migrates as 45-55 kDa due to glycosylation.
>95% as determined by reduced SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) was more than 90% and around 85-100 kDa verified by SEC-MALS.
Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) with a linear range of 0.4-6 ng/mL (QC tested).
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Human CD3 MAb (OKT3) with an affinity constant of 1.12 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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