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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-H52W8

Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) (CDD-H52W8) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified) is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.2 kDa (CD3E) and 37.3 kDa (CD3D). Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with Flag-tagged human IgG1 Fc fragment at the C-terminus. The reducing (R) protein migrates as 45-55 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) (Cat. No. CDD-H52W8) SDS-PAGE gel

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) (Cat. No. ) MALS images

The purity of Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) was more than 90% and around 85-100 kDa verified by SEC-MALS.

Report

Bioactivity-ELISA
Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity)Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) (Cat. No. CDD-H52W8) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) with a linear range of 0.4-6 ng/mL (QC tested).

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity)Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified, Super sensitivity) (Cat. No. CDD-H52W8) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) at 5 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.2-6 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDD-H52W8) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Human CD3 MAb (OKT3) with an affinity constant of 1.12 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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