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Your Position: Home > MabAffinity Chromatography Media > MabAffinity Protein G Agarose High Flow, 100ml

MabAffinity Protein G High Flow Beads,100ml

MabAffinity Protein G High Flow Beads, with rProtein G coupled to high flow Agarose matrix, is a very popular affinity resin for poly and monoclonal antibody/fragments purification and isolation of immune complex. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Compared to protein A, protein G binds more strongly to polyclonal IgG from cow, sheep and horse. Furthermore, unlike protein A, MabAffinity Protein G High Flow Beads can be used successfully to capture polyclonal rat IgG, human IgG3 and mouse IgG1.In addition, MabAffinity Protein G High Flow Beads can in some cases be used for Fab and F(ab)2 purification because of its binding affinity to CH1 domain of certain Fab.
Features
  1. Binding specificities that complement Protein A media

  2. Suitable for different applications, including poly or monoclonal antibodies of different species and subclasses for its broad binding specificity

  3. No albumin binding domain and avoid undesirable interactions with albumin

  4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Specification
CompositionHighly cross-linked Agarose
Average particle size90 micron
FormSlurry in 20% Ethanol
Pack size100ml settled agarose media
(200ml 50% slurry)
LigandrProtein G
Coupling chemistryEpoxy
Dynamic binding capacity1~ 18mg IgG /ml media
Recommended flow rate100-300cm/hr
Chemical stabilityStable in all aqueous buffers commonly used in protein G chromatography
pH working range3-9
pH CIP range (short term)2-10
CIP stability8M Urea, 6M Gua-HCl, 0.1M acetic acid
Temperature stability12-40oC
Storage20% Ethanol
Shelf life5 years
  1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

MabAffinity Protein G High Flow Beads belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity Protein G High Flow Beads are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

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Price(USD) : $1500.00

Inventory : In stock

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