MabAffinity Protein G High Flow Beads,100ml

MabAffinity Protein G High Flow Beads, with rProtein G coupled to high flow Agarose matrix, is a very popular affinity resin for poly and monoclonal antibody/fragments purification and isolation of immune complex. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Compared to protein A, protein G binds more strongly to polyclonal IgG from cow, sheep and horse. Furthermore, unlike protein A, MabAffinity Protein G High Flow Beads can be used successfully to capture polyclonal rat IgG, human IgG3 and mouse IgG1.In addition, MabAffinity Protein G High Flow Beads can in some cases be used for Fab and F(ab)2 purification because of its binding affinity to CH1 domain of certain Fab.

1. Binding specificities that complement Protein A media

2. Suitable for different applications, including poly or monoclonal antibodies of different species and subclasses for its broad binding specificity

3. No albumin binding domain and avoid undesirable interactions with albumin

4. Single point attachment coupling chemistry gives better ligand accessibility for higher binding capacity

Composition	Highly cross-linked Agarose
Average particle size	90 micron
Form	Slurry in 20% Ethanol
Pack size	100ml settled agarose media (200ml 50% slurry)
Ligand	rProtein G
Coupling chemistry	Epoxy
Dynamic binding capacity1	~ 18mg IgG /ml media
Recommended flow rate	100-300cm/hr
Chemical stability	Stable in all aqueous buffers commonly used in protein G chromatography
pH working range	3-9
pH CIP range (short term)	2-10
CIP stability	8M Urea, 6M Gua-HCl, 0.1M acetic acid
Temperature stability1	2-40oC
Storage	20% Ethanol
Shelf life	5 years

1. Delivered at room temperature, and recommended long-term storage at 2-8 °C

MabAffinity Protein G High Flow Beads belongs to the MabAffinity resins family for capture of monoclonal antibodies (MAbs), and is available with different pack sizes for easy Mabs purification, process development and scale up. Immunoprecipitation studies with MabAffinity Protein G High Flow Beads are also commonly used to purify proteins or protein complexes.

For more information and technical questions, Please contact us via TechSupport@acrobiosystems.com.

Cat No	Product Description	Size/ Price
MA-0422-C5	MabAffinity rProtein A Prepacked Column 5ml	5ml ($290)
MA-0422-02	MabAffinity rProtein A High Flow Beads	25ml ($635)
MA-0422-03	MabAffinity rProtein A High Flow Beads	100ml ($1200)
MG-0422-C5	MabAffinity Protein G Prepacked Column 5ml	5ml ($350)
MG-0422-02	MabAffinity Protein G High Flow Beads	25ml ($900)
MG-0422-03	MabAffinity Protein G High Flow Beads	100ml ($1500)
ML-0422-C5	MabAffinity Protein L Prepacked Column 5ml	5ml ($395)
ML-0422-01	MabAffinity Protein L High Flow Beads	5ml ($390)
ML-0422-02	MabAffinity Protein L High Flow Beads	25ml ($1400)
ML-0422-03	MabAffinity Protein L High Flow Beads	100ml ($2900)
FK-0422-C1	FabAffinity KBP Prepacked Column 1ml	1ml ($220)
FK-0422-C5	FabAffinity KBP Prepacked Column 5ml	5ml ($650)
FK-0422-01	FabAffinity KBP High Flow Beads	5ml ($550)