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Your Position: Home > All Other Proteins > IL-2 R gamma > ILG-H85E8

Biotinylated Human IL-2 R gamma / CD132 Protein, His,Avitag™ (SPR verified)

  • Synonym
    IL2RG,CD132,CIDX,IMD4,P64,SCIDX,SCIDX1,gammaC
  • Source
    Biotinylated Human IL-2 R gamma, His,Avitag (ILG-H85E8) is expressed from Baculovirus-Insect cells. It contains AA Leu 23 - Asn 254 (Accession # P31785-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Asn 254) P31785-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 31.0 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) SDS-PAGE gel

Biotinylated Human IL-2 R gamma, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) immobilized on SA Chip can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 105 μM as determined in a SPR assay (Biacore T200) (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Biotinylated Human IL-2 R gamma, His,Avitag (Cat. No. ILG-H85E8) inhibits the IL-2-dependent proliferation of Mo7e cells. The EC50 for this effect is 4.08-5.90 µg/mL in the presence of 10 µg/mL of human IL-2 R beta and 50 ng/mL of human IL-2 (Routinely tested).

  • Background
    IL-2R is a heterotrimeric protein binds and responds to the cytokine IL-2. Three distinct chains of IL-2R, termed as α, β and γ, which are non-covalently associated are identified. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ chain, along with the β subunit. The α chain of the IL-2R can bind to the β chain before receptor interaction with IL-2. The γ chain alone has a very weak affinity for IL-2, but after the ligand is bound to the α/β heterodimer, the γ chain becomes recruited to the complex to form a very stable macromolecular quaternary ligand/receptor complex. Interleukin-2 receptor subunit gamma (IL2RG), also known as cytokine receptor common subunit gamma, CD antigen CD132, gammaC, p64, which belongs to the type I cytokine receptor family or type 5 subfamily. IL2RG is located on the surface of immature blood-forming cells in bone marrow. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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