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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-C52W6

Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (MALS verified)

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (CDG-C52W6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1(CD3E) & Q95LI7-1(CD3G)).
    Predicted N-terminus: Gln 22 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)) Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)

    Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.0 kDa (CD3E) and 14.4 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 18-22 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) SDS-PAGE gel

Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. ) MALS images

The purity of Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Tag Free (Cat. No.CDG-C52W6) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag FreeCynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) ELISA bioactivity

Immobilized Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free(Cat. No. CDG-C52W6) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated Human BCMA, Fc,Avitag™, (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.5 ng/mL (Intact assay, QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD3×BCMA scFV on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) with an affinity constant of 6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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