Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.0 kDa (CD3E) and 14.4 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 18-22 kDa due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Tag Free (Cat. No.CDG-C52W6) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Immobilized Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free(Cat. No. CDG-C52W6) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) and then add Biotinylated Human BCMA, Fc,Avitag™, (Cat. No. BC7-H82F0) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.5 ng/mL (Intact assay, QC tested).
Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human CD3×BCMA scFV on AHC Biosensor via DMF Filed Human BCMA, Fc Tag (Cat. No. BC7-H5254), can bind Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-C52W6) with an affinity constant of 6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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