This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated SARS-CoV-2 Spike RBD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) was more than 90% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS.
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 4-125 ng/mL (QC tested).
Loaded Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) on SA Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zupancic JM, Desai AA, Schardt JS, et al.
Journal: Cell chemical biology 2021
Application: Binding Assay
Authors: Troyer, Zach et al.
Journal: Journal of extracellular vesicles vol 2021
Authors: Mei, Jie et al.
Journal: Journal of ethnopharmacology 2021
Authors: Pratik Adhikary, Sashi Kandel, et al.
Journal: Advanced Therapeutics. 2021
Authors: Keating SM, Mizrahi RA, Adams MS, et al.
Journal: Nature biotechnology 2021
Application: Flow cytometry
Authors: Esparza, Thomas J et al.
Journal: Scientific reports 2020
Authors: Guo L, Yao Z, Yang L, et al.
Journal: Chemical engineering journal 2020
Application: SPR & ELISA
Authors: Mulligan, Mark J et al.
Journal: Nature 2020
Application: Immunogenicity assessments
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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