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Your Position: Home > All Other Proteins > GUCY2C > GUC-H5257

Human GUCY2C / Guanylyl cyclase C Protein, Fc Tag

  • Synonym
    GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
  • Source
    Human GUCY2C, Fc Tag (GUC-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
    Predicted N-terminus: Ser 24
  • Molecular Characterization
    Online(Ser 24 - Gln 430) P25092-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) SDS-PAGE gel

Human GUCY2C, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human GUCY2C, Fc TagHuman GUCY2C, Fc Tag (Cat. No. GUC-H5257) ELISA bioactivity

Immobilized Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc and analyzed with FACS (Routinely tested).

  • Background
    GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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