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Your Position: Home > All Other Proteins > GUCY2C > GUC-H52H5

Human GUCY2C / Guanylyl cyclase C Protein, His Tag

  • Synonym
    GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C
  • Source
    Human GUCY2C, His Tag (GUC-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).
    Predicted N-terminus: Ser 24
  • Molecular Characterization
    Online(Ser 24 - Gln 430) P25092-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human GUCY2C, His Tag (Cat. No. GUC-H52H5) SDS-PAGE gel

Human GUCY2C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human GUCY2C, His TagHuman GUCY2C, His Tag (Cat. No. GUC-H52H5) ELISA bioactivity

Immobilized Human GUCY2C, His Tag (Cat. No. GUC-H52H5) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, His Tag (Cat. No. GUC-H52H5) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).

  • Background
    GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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