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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-H82E5

Biotinylated Human / Cynomolgus / Rhesus macaque CD28 Protein, His,Avitag™

  • Synonym
    CD28,Tp44
  • Source
    Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (CD8-H82E5) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1).
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) P10747-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 18.7 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) SDS-PAGE gel

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) ELISA bioactivity

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) with a linear range of 0.2-5 ng/mL (QC tested).

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) ELISA bioactivity

Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-2, Fc Tag (Cat. No. CD6-H5257) with a linear range of 2-78 ng/mL (Routinely tested).

Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,AvitagBiotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) ELISA bioactivity

Immobilized Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag (Cat. No. CD8-H82E5) with a linear range of 0.1-3 ng/mL (Routinely tested).

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Price(USD) : $250.00

Price(USD) : $980.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:15 Details
  • Latest Research Phase:Phase ?

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