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Your Position: Home > Viral Proteins > S2 protein > S2N-C52H5
  • Synonym
    Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2,COVID-19
  • Source
    SARS-CoV-2 S2 protein, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production. 
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.0 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H5) SDS-PAGE gel

SARS-CoV-2 S2 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
SARS-CoV-2 S2 protein, His TagSARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H5) ELISA bioactivity

Immobilized SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H5) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 18: Prefusion Coronavirus Spike Proteins
    This product and its use is the subject of one or more U.S. Provisional Patent Application Nos. 62/412,703; 62/972,886; 63/032,502; U.S. Patent Application No. 16/344,774 and corresponding patents and applications in other countries. This product is sold under licenses from The National Institute of Allergy & Infectious Diseases/National Institutes of Health and The University of Texas at Austin. Rights to use this product are limited to research only (including Non-clinical Diagnostic Kits), and expressly exclude the right to use in human therapeutics, vaccines and clinical diagnostics and any use involving the administration of this product to humans. The purchase of this product conveys to the buyer the non-transferrable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for other purposes shall be direct to NIAID/NIH, Technology Transfer and Intellectual Property Office, 5601 Fisher Lane, Suite 6D, Rockville, MD 20852-3804; and The University of Texas at Austin, MCC Building, Mail Code R3500, 3925 West Braker Lane, Suite 1.9A, Austin, Texas 78759.

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