This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.4 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CTLA-4, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CTLA-4, His Tag (MALS verified) (Cat. No. CT4-H52Hb) was more than 90% in HP-SEC, and around 40-55 kDa verified by SEC-MALS.
Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (MALS verified) (Cat. No. CT4-H52Hb) with a linear range of 0.2-2 ng/mL (QC tested).
Yervoy (Ipilimumab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CTLA-4, His Tag (MALS verified) (Cat. No. CT4-H52Hb) with an affinity constant of 55.2 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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high quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
CD24 protein with different tags and CD24 overexpressed cells, The binding activity of CD24 overexpressed cells and Siglec-10 was verified by FACS.
Enzyme activity＞37000 pmol/min/μg. Purity is greater than 90%.
Bioactivity is verified by BLI/SPR/ELISA.
HEK293 expressed full length CD20 protein with Nanodisc/DDM. Complete conformation.High affinity(nM) of RTX/OFA and CD20 protein.
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