|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Coupled amount of protein||>318 pmol (25 μg) S1 protein / mg Beads||>318 pmol (25 μg) S1 protein / mg Beads|
|Capacity||>40 μg anti-SARS2-CoV-2 S1 antibody / mg beads; >20 μg ACE2 protein / mg beads||>40 μg anti-SARS2-CoV-2 S1 antibody / mg beads; >20 μg ACE2 protein / mg beads|
|Formulation||PBS, 0.05% Tween-20, pH7.4, with 10% Trehalose||PBS, 0.05% Tween-20, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads / mL)||Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)|
|Concentration||1 mg beads / mL||1 mg beads / mL|
Upon receipt, please store the lyophilized beads at -20℃ for long term storage. The shelf life is 1 year at -20℃ in lyophilized form.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 1.003 μg/mL (QC tested).
Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.025 μg/mL (QC tested).
The binding curves between SARS-CoV-2 S1 pre-coupling magnetic beads (Cat. No. MBS-K001) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation,including proteins, antibodies and kits. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection.
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed a serial of high-quality antigen and antibody reagents, including super stable trimeric S protein, S RBD, S1, S2, N proteins mainly expressed by HEK 293 and paired antibodies with outstanding sensitivity verified by inactivated virus samples.
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