| Items | Size | Size |
|---|---|---|
| Beads Size | 2 mg | 10 mg |
| Particle size | 2 μm | 2 μm |
| Coupled amount of RBD protein | 80 μg | 400 μg |
| Capacity | > 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads | > 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose | Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4 , with 10% trehalose |
| Reconstitution | 2 mL ultrapure water (1mg beads/mL) | 10 mL ultrapure water (1mg beads/mL) |
Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately.
Do not to freeze thaw the Beads after reconstitution.
Application Method:
a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 0.8887 μg/mL (QC tested).
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.008 μg/mL (QC tested).
The binding curves between SARS-CoV-2 S RBD pre-coupling magnetic beads (Cat. No. MBS-K002) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation,including proteins, antibodies and kits. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection.
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed a serial of high-quality antigen and antibody reagents, including super stable trimeric S protein, S RBD, S1, S2, N proteins mainly expressed by HEK 293 and paired antibodies with outstanding sensitivity verified by inactivated virus samples.
ACROBiosystems has developed a series of kit products using its high-quality SARS-CoV-2 antigen proteins to improve the efficiency of anti-SARS-CoV-2 drugs and vaccines development.
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