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Your Position: Home > Kits > SARS-CoV-2 S1 > MBS-K002

SARS-CoV-2 (COVID-19) Spike protein RBD-coupled magnetic beads

For research use only.
Materials Provided
ItemsSize Size
Beads Size2 mg10 mg
Particle size2 μm2 μm
Coupled amount of RBD protein80 μg400 μg
Capacity> 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads > 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads
FormulationLyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehaloseLyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4 , with 10% trehalose
Reconstitution2 mL ultrapure water (1mg beads/mL)10 mL ultrapure water (1mg beads/mL)
  • Background
    The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
  • Application
    This product is intended for immunocapture, cell stimulating, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately.

    Do not to freeze thaw the Beads after reconstitution.

  • Assay Principles
    The Antigen pre-Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. We provide the SARS-CoV-2 Spike protein RBD coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as Cell stimulating, biopanning or Flow cytometric analysis.

    Application Method:

    a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.

    b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.

Typical Data
SARS-CoV-2 Spike protein RBD-coupled magnetic beadsSARS-CoV-2 Spike protein RBD-coupled magnetic beads (Cat. No. MBS-K002) ELISA bioactivity

Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 0.8887 μg/mL (QC tested).

SARS-CoV-2 Spike protein RBD-coupled magnetic beadsSARS-CoV-2 Spike protein RBD-coupled magnetic beads (Cat. No. MBS-K002) ELISA bioactivity

Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.008 μg/mL (QC tested).

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