|Beads Size||2 mg||10 mg|
|Particle size||2 μm||2 μm|
|Coupled amount of RBD protein||80 μg||400 μg|
|Capacity||> 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads||> 200 nmol / mg beads > 40 μg anti-SARS2-CoV-2 S1 antibody / mg beads > 40 μg ACE2 protein / mg beads|
|Formulation||Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4, with 10% trehalose||Lyophilized from 0.22 μm filtered solution in PBS, 0.05% Tween-20, pH7.4 , with 10% trehalose|
|Reconstitution||2 mL ultrapure water (1mg beads/mL)||10 mL ultrapure water (1mg beads/mL)|
Upon receipt, please store the Beads at -20℃ for 1 year in lyophilized state. Once the Beads reconstitution, please use it immediately.
Do not to freeze thaw the Beads after reconstitution.
a) Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.
b) Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 0.8887 μg/mL (QC tested).
Immobilized 40 μg SARS-CoV-2 S protein RBD to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.008 μg/mL (QC tested).
The binding curves between SARS-CoV-2 S RBD pre-coupling magnetic beads (Cat. No. MBS-K002) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).
ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation,including proteins, antibodies and kits. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection.
High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed a serial of high-quality antigen and antibody reagents, including super stable trimeric S protein, S RBD, S1, S2, N proteins mainly expressed by HEK 293 and paired antibodies with outstanding sensitivity verified by inactivated virus samples.
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