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Your Position: Home > All Other Proteins > Streptavidin > STN-NH115

Streptavidin-HRP

  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin is expressed from E.coli cells and conjugated with horseradish peroxidase under optimal conditions.
  • Molecular Characterization

    This protein .

  • Application
    Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry (1-10 µg/mL), and western blot (2 µg/mL) applications.
    Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
    NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.
  • Endotoxin
  • Formulation

    Lyophilized from 0.22 μm filtered solution in Na3PO4 and NaCl pH6.8. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 2 years in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
Streptavidin-HRPStreptavidin-HRP (Cat. No. STN-NH115) ELISA bioactivity

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Avitag,His Tag (Cat. No. PD1-H82E4) with a linear range of 0.8-13 ng/mL when detected by Streptavidin-HRP (Cat. No. STN-NH115) (Routinely tested).

Streptavidin-HRPStreptavidin-HRP (Cat. No. STN-NH115) ELISA bioactivity

Serial dilutions of SARS-CoV-2 inhibitor (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions detected by Streptavidin-HRP (Cat. No. STN-NH115) (Routinely tested).

  • Background
    Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Order Details

    Price(USD) : $200.00

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    DMF (Drug Master File)

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