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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-H82W6

Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (CDD-H82W6) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and an Avi tag (Avitag™) at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 20-28 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) SDS-PAGE gel

Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ) MALS images

The purity of Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) was more than 85% and around 40-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) ELISA bioactivity

Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) (Cat. No. CDE-M120a) with a linear range of 0.2-6 ng/mL (QC tested).

Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) ELISA bioactivity

Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind UCHT1 with a linear range of 0.1-3 ng/mL (Routinely tested).

Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) ELISA bioactivity

Quantitative Analysis of Bispecific T-cell Engager (CD3 X BCMA) in human serum.
Immobilized Human BCMA, His Tag (Cat. No. BCA-H522y) at 2 μg/mL, add increasing concentrations of Bispecific T cell Engager (CD3 X BCMA) in 10% human serum and then add Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 15 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) captured on Biotin CAP - Series S sensor Chip can bind Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 22.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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