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Your Position: Home > All Other Proteins > CD3 epsilon > CDE-H5223

Human CD3 epsilon Protein, His Tag

  • Synonym
    FLJ18683,T3E,TCRE,CD3E,CD3-epsilon
  • Source
    Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # NP_000724.1).
    Predicted N-terminus: Asp 23
  • Molecular Characterization
    Online(Asp 23 - Asp 126) NP_000724.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 12.6 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) SDS-PAGE gel

Human CD3 epsilon, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human CD3 epsilon, His TagHuman CD3 epsilon, His Tag (Cat. No. CDE-H5223) ELISA bioactivity

Immobilized Mouse Anti-Human CD3 (SP34-2) at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with a linear range of 0.4-6.25 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.97 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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