This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.6 kDa. The protein migrates as 15-16 kDa under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD3 epsilon, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.97 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Authors: TJ Schuetz, et al
Journal: US20190031785A1 43496
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